Activation Of α7 Nicotinic Acetylcholine Receptors Prevents Monosodium Iodoacetate-Induced Osteoarthritis In Rats

Background/Aims: Although some evidence suggests that the prevalence of osteoarthritis (OA) is lower in smokers compared to nonsmokers, the mechanisms of nicotine-induced protection remain unclear. Stimulation of the α7 nicotinic acetylcholine receptor (α7-nAChR) appears to be a critical mechanism underlying the anti-inflammatory potential of cholinergic agonists in immune cells. The inhibition of secreted inflammatory molecules and the subsequent inflammatory processes have been proposed as a novel strategy for the treatment of OA. The objective of the present study was to determine whether nicotine-induced protection in a monosodium iodoacetate (MIA) rat model of OA occurs via α7-nAChR-mediated inhibition of chondrocytes. Methods: Both in vivo (MIA) and in vitro (MIA; Interleukin-1β, IL-1β) models of OA were used to investigate the roles and the possible mechanisms whereby α7-nAChRs protect against knee joint degradation. Multiple experimental approaches, including macroscopic, histological analysis, chondrocyte cell cultures, confocal microscopy, and western blotting, were employed to elucidate the mechanisms of α7-nAChR-mediated protection. Results: Systemic administration of nicotine alleviated MIA-induced joint degradation. The protective effects of nicotine were abolished by administration of the α7-nAChR-selective antagonist methyllycaconitine (MLA). In primary cultured rat chondrocytes, pretreatment with nicotine suppressed both p38, extracellular regulated kinase (Erk) 1/2 and c-Jun-N-terminal kinase (JNK) mitogen-activated protein kinases (MAPK) phosphorylation and phosphorylated nuclear factor-kappa B (NF-κB) p65 activation induced by MIA- or IL-1β, and these effects were also reversed by MLA. Conclusion: Taken together, our results suggest that activation α7-nAChRs is an important mechanism underlying the protective effects of nicotine

Multipoint-Interconnected Quantum Communication Networks

As quantum computers with sufficient computational power are becoming mature, the security of classical communication and cryptography may compromise, which is based on the mathematical complexity. Quantum communication technology is a promising solution to secure communication based on quantum mechanics. To meet the secure communication requirements of multiple users, multipoint-interconnected quantum communication networks are specified, including quantum key distribution networks and quantum teleportation networks. The enabling technologies for quantum communication are the important bases for multipoint-interconnected quantum communication networks. To achieve the better connection, resource utilization, and resilience of multipoint-interconnected quantum communication networks, the efficient network architecture and optimization methods are summarized, and open issues in quantum communication networks are discussed

Structures and Low Dimensional Classifications of Hom-Poisson Superalgebras

Hom-Poisson superalgebras can be considered as a deformation of Poisson superalgebras. We prove that Hom-Poisson superalgebras are closed under tensor products. Moreover, we show that Hom-Poisson superalgebras can be described using only the twisting map and one binary operation. Finally, all algebra endomorphisms on 2-dimensional complex Poisson superalgebras are computed, and their associated Hom-Poisson superalgebras are described explicitly

Simple Modules for Modular Lie Superalgebras W(0∣n), S(0∣n), and K(n)

This paper constructs a series of modules from modular Lie superalgebras W(0∣n), S(0∣n), and K(n) over a field of prime characteristic p≠2. Cartan subalgebras, maximal vectors of these modular Lie superalgebras, can be solved. With certain properties of the positive root vectors, we obtain that the sufficient conditions of these modules are irreducible L-modules, where L=W(0∣n), S(0∣n), and K(n)

Effects of Sterilization Methods on the Changes in Fatty Acid and Volatile Compounds of Oil-impregnated Soft Canned Saury during Storage

This study investigated the potential relationships between the composition changes of fatty acid and volatile flavor substances in soft canned saury in olive oil which treated with three various sterilization techniques including: Ultra-high pressure sterilization (UHPSO), traditional sterilization (RSO) and microwave-assisted sterilization (MTSO). These results showed that fatty acids in olive oil infiltrated into the fish, especially the unsaturated fatty acids migrate into the meat, improved its nutritional quality. The MTSO group owned the best storage quality, while the UHPSO group performed the worst. Aldehydes, alcohols, and hydrocarbons were the main sources of volatile compounds in soft canned saury in oil in the three treatment groups, with aldehydes accounting for a large proportion and being the main contributors to the aroma (e.g. hexanal, nonanal, tridecanal, etc.). Changes in aldehyde compounds were strongly correlated with oleic acid and linoleic acid content. Olive oil treatment could effectively reduce the content of fishy odor compounds in saury (e.g. 1-octen-3-ol, trimethylamine, etc.). With increasing of storage time, the variety and richness of volatile compounds in soft canned saury in oil in UHPSO group and MTSO group displayed a trend of first rising and then decreasing, while aldehydes substances in RSO group kept increasing. In summary, oil immersion treatment could improve the nutritional quality of saury flesh, and the MTSO group had more flavor substance during storage, provide a theoretical basis for the processing and treatment of saury food products

High Expression of XRCC6 Promotes Human Osteosarcoma Cell Proliferation through the β-Catenin/Wnt Signaling Pathway and Is Associated with Poor Prognosis

Increasing evidences show that XRCC6 (X-ray repair complementing defective repair in Chinese hamster cells 6) was upregulated and involved in tumor growth in several tumor types. However, the correlation of XRCC6 and human osteosarcoma (OS) is still unknown. This study was conducted with the aim to reveal the expression and biological function of XRCC6 in OS and elucidate the potential mechanism. The mRNA expression level of XRCC6 was measured in osteosarcoma cells and OS samples by quantitative transcription-PCR (qRT-PCR). The expression of XRCC6 protein was measured using Western blot and immunohistochemical staining in osteosarcoma cell lines and patient samples. Cell Counting Kit 8 (CCK8), colony-forming and cell cycle assays were used to test cell survival capacity. We found that XRCC6 was overexpressed in OS cells and OS samples compared with the adjacent non-tumorous samples. High expression of XRCC6 was correlated with clinical stage and tumor size in OS. Reduced expression of XRCC6 inhibits OS cell proliferation through G2/M phase arrest. Most importantly, further experiments demonstrated that XRCC6 might regulate OS growth through the β-catenin/Wnt signaling pathway. In conclusion, these findings indicate that XRCC6 exerts tumor-promoting effects for OS through β-catenin/Wnt signaling pathway. XRCC6 may serve as a novel therapeutic target for OS patients

Genome-Wide Analysis of TCP Transcription Factors and Their Expression Pattern Analysis of Rose Plants (<i>Rosa chinensis</i>)

The plant-specific transcription factor TEOSINTE BRANCHED, CYCLOIDEA, AND PROLIFERATING CELL FACTOR (TCP) gene family plays vital roles in various biological processes, including growth and development, hormone signaling, and stress responses. However, there is a limited amount of information regarding the TCP gene family in roses (Rosa sp.). In this study, we identified 18 TCP genes in the rose genome, which were further classified into two subgroups (Group A and Group B) via phylogenetic analysis. Comprehensive characterization of these TCP genes was performed, including gene structure, motif composition, chromosomal location, and expression profiles. Synteny analysis revealed that a few TCP genes are involved in segmental duplication events, indicating that these genes played an important role in the expansion of the TCP gene family in roses. This suggests that segmental duplication events have caused the evolution of the TCP gene family and may have generated new functions. Our study provides an insight into the evolutionary and functional characteristics of the TCP gene family in roses and lays a foundation for the future exploration of the regulatory mechanisms of TCP genes in plant growth and development

Overexpression of X-Box Binding Protein 1 (XBP1) Correlates to Poor Prognosis and Up-Regulation of PI3K/mTOR in Human Osteosarcoma

Increasing evidence demonstrates that dysregulation of XBP1 function contributes to tumorigenesis in some cancers. However, little is known about the role of XBP1 in the progression of osteosarcoma (OS). The expression of XBP1 in OS samples was measured by quantitative RT-PCR and Western blotting assays. Cell cycle analysis and cell counting kit 8 (CCK8) assays were performed to determine the effects of XBP1 expression on cells growth capacity. Cell apoptosis coassay was applied to determine cell survival. The expression of genes affected by XBP1 was examined by quantitative RT-RCR and validated by Western blotting assays. XBP1 was overexpressed in OS clinical samples compared with corresponding non-cancerous tissues. Overexpression of XBP1 was significantly associated with advanced clinical stages, high degree of malignancy and low tumor necrosis rate. Furthermore, hypoxia activated XBP1, and silencing XBP1 significantly enhanced OS cell apoptosis. Knock-down of XBP1 resulted in inhibition of OS growth. Most importantly, knockdown of XBP1 led to down-regulation of PIK3R3 and mTOR. Taken together, XBP1 is up-regulated and has a pro-tumor effect in OS with activation of PI3K/mTOR signaling. Thus, targeting XBP1 may provide a new potential therapeutic method for OS